![]() The capsules are impermeable to dyes and thus must be view with a Negative stain. These capsules hide the microbe from the immune system and are also used as a "glue" to keep the microbe from being swallowed with the saliva. No SMEAR is made before staining and thus the microbe is alive and experiences no shrinkage from preparation.Ĭertain microbes in the mouth use sticky sugar capsules to attach themselves to teeth. This is NOT really a stain as the dyes do not penetrate the microbe, instead the obliterate the background leaving the microbes translucent and visible in a darkened field. As bacteria are colorless, this procedure involved mixing the microbes with NIGROSINE ( India Ink - is less useful). The simplest way to prepare bacteria for staining involves NEGATIVE or BACKGROUND STAINING. NEGATIVE (Stain?) can be used as a Capsule Stain see Atlas page 31 Text: 21 The same as above regarding the info 1st in the text Thanks for taking my class! if you are dedicated and keep trying I will "drag you" to success in Microbiology 20!Įmails: in the Subject put last name 1st ID # and a "hook" that indicates the question OPTIONAL: a box of Nitrile Gloves (that fit you) a broad/wide point (NOT FINE POINT) BLACK SHARPEE that clicks closed/no cap. YOU DO NOT NEED TO BUY LAB FACEMASKS,FACE SHIELDS,or a LAB MANUAL AS WE PROVIDE THEM for you as "loaner materials." Buy a binder for 3 holed papers - NOT A BINDER WITH METAL RINGS, 1 the can hold 1 inch of handouts and worksheets ands has metal tabs the you "bend" to close it. Buy a can of "Bon Ami" scouring powder (or "Bartender's Friend" NO OTHER BRANDS, in supermarkets near Ajax etc.ħ. the more cotton in the coat the better it "breaths" the more poly material the more you will sweat.Ĥ. ![]() REMEMBER BECAUSE OF BUNSEN BURNERS, IT GETS HOT IN LAB EVEN WITH A/C. Buy a long sleeve and long torso (to the knee area) WHITE cotton or cotton mix Lab coat (on Amazon for $10 and up). ![]() Go to the LACC Bookstore and purchase the Microbiology-20 Lab Packet.Ģ. This is OYO (On your own - no lecture but it will be expected).ġ. Inside MicroDropbox go to "Tests & Ancillary Materials." Download a copy of an old edition of our Textbook "Microbiology: Principles & Explorations" by Black and read the textbook Introduction, Chapter 1 "Why Study Microbiology" & Chapter 2 a review of Chemistry - dont panic! We just need you to know basics like: atoms, elements, the Periodic Chart, bonds, molecules & compounds. "My philosophy of Education & How to get an "A" in Microbiology" then move down the page & click on the BLUE button for the MicroDropBox. Lecture: YOU DO NOT NEED TO BUY A TEXTBOOK or LAB MANUAL! Go to YouTube and subscribe to our hicksmicro YouTube site. Until then there are a few things that you could do now to prepare some of them may be found on my "old" website" at (ignore any not safe or unsafe "site" notifications). Please peruse the website (click on the links) and look over how the course is organized: HINT: BY EXAM number. Monday, you will receive an email alerting you that our Syllabus and Canvas sight is active. ![]()
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